Summary | Detection of specific Bovine Tuberculosis(BTB) antibody |
Principle |
Bovine Tuberculosis(BTB) antibody Elisa test kit can be used to detect Bovine Tuberculosis antibody in serum or plasma of Bovine. |
Detection Targets | Bovine Tuberculosis(BTB) antibody |
Sample | Serum
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Quantity | 1 kit = 192 Test |
Stability and Storage |
1) All reagents should be stored at 2~8℃. Do not freeze. 2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.
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Mycobacterium bovis is a slow-growing (16- to 20-hour generation time) aerobic bacterium and the causative agent of tuberculosis in cattle (known as bovine TB). It is related to Mycobacterium tuberculosis, the bacterium which causes tuberculosis in humans. M. bovis can jump the species barrier and cause tuberculosis-like infection in humans and other mammals.
This kit use indirect ELISA method, pured BTB antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is BTB specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme conjugate, discard the uncombined enzyme conjugate with
washing. Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is directly proportion of antibody content in sample.
Reagent |
Volume 96 Tests/192Tests |
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1 |
|
1ea/2ea |
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2 |
|
2.0ml |
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3 |
|
1.6ml |
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4 |
|
100ml |
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5 |
|
100ml |
|
6 |
|
11/22ml |
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7 |
|
11/22ml |
|
8 |
|
15ml |
|
9 |
|
2ea/4ea |
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10 | serum dilution microplate |
1ea/2ea |
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11 | Instruction |
1 pcs |