Summary | Detection of specific Antibody of Foot and MouthDisease Type The BRU |
Principle |
Principle of the Test Brucellosis(Bru) antibody Elisa test kit can be used to detect Brucellosis antibody in serum or plasma of Bovine and Pig. |
Detection Targets | The BRU antibody |
Sample | Serum |
Quantity | 1 kit = 192 Test |
Stability and Storage |
1) All reagents should be stored at 2~8℃. Do not freeze. 2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.
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This kit use indirect ELISA method, pured Bru antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is Bru specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme conjugate, discard the uncombined enzyme conjugate with
washing. Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is directly proportion of antibody content in sample.
Reagent |
Volume 96 Tests/192Tests |
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1 |
|
1ea/2ea |
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2 |
|
2ml |
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3 |
|
1.6ml |
|
4 |
|
100ml |
|
5 |
|
100ml |
|
6 |
|
11/22ml |
|
7 |
|
11/22ml |
|
8 |
|
15ml |
|
9 |
|
2ea/4ea |
|
10 | serum dilution microplate |
1ea/2ea |
|
11 | Instruction |
1 pcs |