Summary | Detection of specific Foot and Mouth Disease Type A antibody |
Principle |
The FMD Type A antibody ELISA test kit use to detection of foot-and-mouth disease virus antibodies in the serum of pigs, cattle, sheep and goat for the evaluate of FMD vaccine immunity. |
Detection Targets | Foot and Mouth Disease Type A Antibody |
Sample | Serum
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Quantity | 1 kit = 192 Test |
Stability and Storage |
1) All reagents should be stored at 2~8℃. Do not freeze. 2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.
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Foot-and-mouth disease virus (FMDV) is the pathogen that causes foot-and-mouth disease. It is a picornavirus, the prototypical member of the genus Aphthovirus. The disease, which causes vesicles (blisters) in the mouth and feet of cattle, pigs, sheep, goats, and other cloven-hoofed animals is highly infectious and a major plague of animal farming.
This kit use competitive ELISA method to pre-coated foot-and-mouth disease virus antigens on microplate wells. When testing, add diluted serum sample and anti-FMD Ab, after incubation, if there have FMD antibody, it will combine with the pre-coated antigen, antibody in sample block the combination of anti-FMD antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample.
Foot-and-mouth disease virus occurs in seven major serotypes: O, A, C, SAT-1, SAT-2, SAT-3, and Asia-1. These serotypes show some regionality, and the O serotype is most common.
Reagent |
Volume 96 Tests/192Tests |
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1 |
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1ea/2ea |
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2 |
|
2.0ml |
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3 |
|
1.6ml |
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4 |
|
100ml |
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5 |
|
100ml |
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6 |
|
11/22ml |
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7 |
|
11/22ml |
|
8 |
|
15ml |
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9 |
|
2ea/4ea |
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10 | serum dilution microplate |
1ea/2ea |
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11 | Instruction |
1 pcs |