Summary | Detection of specific Antibody of Flu A immune and serological diagnosis of infection in Avian, swine and Equus. |
Principle |
Influenza A antibody Elisa kit is used to detect specific antibody against Influenza A Virus (Flu A) in serum, for monitoring antibody after Flu A immune and serological diagnosis of infection in Avian, swine and Equus.
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Detection Targets | Influenza A antibody |
Sample | Serum
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Quantity | 1 kit = 192 Test |
Stability and Storage |
1) All reagents should be stored at 2~8℃. Do not freeze. 2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.
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Influenza A virus is a pathogen that causes influenza in birds and some mammals. It is an RNA virus, subtypes of which have been isolated from wild birds. Occasionally, it spreads from wild birds to poultry, which can lead to severe disease, outbreaks, or human influenza pandemics
This kit use block ELISA method, FluA antigen is pre-coated on microplate. When testing, add diluted serum sample, after incubation, if there is Flu A specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-Flu A monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing. Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample.
Reagent |
Volume 96 Tests/192Tests |
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1 |
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1ea/2ea |
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2 |
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2.0ml |
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3 |
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1.6ml |
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4 |
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100ml |
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5 |
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100ml |
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6 |
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11/22ml |
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7 |
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11/22ml |
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8 |
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15ml |
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9 |
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2ea/4ea |
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10 | serum dilution microplate |
1ea/2ea |
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11 | Instruction |
1 pcs |