Summary | Detection of specific Antibody of Peste Des Petits Ruminants |
Principle | The PPRV antibody ELISA test kit use to detection of Peste des petits ruminants virus antibodies in the serum of sheep and goat . |
Detection Targets | PPRV antibody |
Sample | Serum
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Quantity | 1 kit = 192 Test |
Stability and Storage |
1) All reagents should be stored at 2~8℃. Do not freeze. 2) Shelf life is 12 months. Use all reagents before the expiry date on the kit.
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Ovine rinderpest, also commonly known as peste des petits ruminants (PPR), is a contagious disease primarily affecting goats and sheep; however, camels and wild small ruminants can also be affected. PPR is currently present in North, Central, West and East Africa, the Middle East, and South Asia. It is caused by small ruminants morbillivirus in the genus Morbillivirus, and is closely related to, among others, rinderpest morbillivirus, measles morbillivirus, and canine morbillivirus (previously known as canine distemper virus). The disease is highly contagious, and can have an 80–100% mortality rate in acute cases in an epizootic setting. The virus does not infect humans.
PPR is also known as goat plague, kata, syndrome of stomatitis-pneumoenteritis, and ovine rinderpest.
Official agencies such as the FAO and OIE use the French name "peste des petits ruminants" with several spelling variants.
This kit use competitive ELISA method to pre-coated PPRV antigens on microplate wells. When testing, add diluted serum sample, after incubation, if there have PPRV antibody, it will combine with the pre-coated antigen, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample.
Reagent |
Volume 96 Tests/192Tests |
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1 |
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1ea/2ea |
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2 |
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2ml |
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3 |
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1.6ml |
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4 |
|
100ml |
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5 |
|
100ml |
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6 |
|
11/22ml |
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7 |
|
11/22ml |
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8 |
|
15ml |
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9 |
|
2ea/4ea |
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10 | serum dilution microplate |
1ea/2ea |
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11 | Instruction |
1 pcs |